Sam Winchester (Padalecki) has done his best to escape his family's eerie history. Unlike his older brother, Dean (Ackles), Sam rejected their father's obsessive quest to uncover the evil forces that claimed the life of the boys' mother. Putting as much distance as possible between himself and his family, Sam left home right after high school and went away to college. His new life included a beautiful and loving girlfriend and plans to enter law school. Dean, however, took a very different path, following their father into the "family business." When Dean arrived on Sam's doorstep with the news that their father has vanished, Sam had no choice but to put his personal plans on hold and join Dean on the road.
Bound by tragedy and blood to their dangerous, otherworldly mission, the brothers travel from the Colorado wilderness to the Nebraska farmlands and on to the isolated Wisconsin lakes, encountering creatures that most people believe only exist in folklore, superstition and nightmares: the terrifying Bloody Mary, the brutal Native American beast known as the Wendigo, the man with a hook who haunts lovers' lanes and the dark phantom travelers who portend a violent plane crash. As they follow the clues in their father's journal, the brothers realize that he has passed on to them all the knowledge he accumulated during the past 20 years. What they can't understand is why he has vanished and left them to carry on without him.
Taking up the mantle of their father's crusade, Sam and Dean crisscross the lonely and mysterious back roads of the country in their '67 Chevy Impala, searching for their missing father--and hunting down every evil supernatural force they encounter along the way.